Please contact the core for generating the online instrument reservation account. Cell sorter is operated by dedicated core facility staff only. If the user already has experience in operation of the instrument, a 5-10 minutes briefing sessions about the general maintenance of the analyzers would be given. Facilities/InstrumentsĮmail Caisheng Lu (CCTI Site) to discuss which instrument best fits the experiment and training will be arranged accordingly. Please contact the facility staff for any issues regarding training, assistance and access with flow including protocol design, instrument operation, data analysis, and troubleshooting. Among the many documented applications, characterization, and quantification of cells for surface protein, cytokine, intracellular transcription factor, and signaling molecules are the most common experiments performed in the flow cytometry core facility. The Flow Cytometry Core of the Columbia Center for Translational Immunology (CCTI) and Herbert Irving Comprehensive Cancer Center (HICCC) provides training and access to state-of-the-art flow cytometry for biomedical investigators at the Columbia University Irving Medical Center. The ability to design, perform and analyze data from multi-parametric flow cytometric experiments requires technical expertise but also immunological expertise to appropriately design the experiment. Flow cytometry has become the primary tool for the identification of cell populations according to specific parameters, and is therefore employed by an ever-growing number of biomedical scientists.
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